Ex-vivo organ slices derived from human surgically resected organs serve as short-term in-vitro models and alternatives (3R’s) to animal experiments. These models offer advantages over classical 2D cell culture systems and organoid models by preserving the functional properties, the cellular heterogeneity and the structural architecture of the original organ for a defined period.
The generation of PCOS from human specimens can be instrumental in addressing specific research questions across various applications, including:
- Host-pathogen interaction studies (focus and experience on human respiratory viruses)
- Testing of antivirals and small molecules
- Drug screening, drug metabolism and toxicity
Standard histology and molecular biology methods can be applied to PCOS as read-outs, including immunohistochemical staining, gene expression analyses, and protein analyses.
